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This is the website for the online book “Orchestrating Spatially-Resolved Transcriptomics Analysis with Bioconductor” (OSTA).

This book provides several examples of computational analysis workflows for spatially resolved-transcriptomics (SRT) data, using the Bioconductor framework within the R programming language. The chapters contain details on individual analysis steps as well as complete workflows, with example datasets and R code that can be run on your own laptop.

The book is organized into several parts, including background, preprocessing steps, analysis steps, and complete workflows.

Additional introductory material on R and Bioconductor can also be found in the related book Orchestrating Single-Cell Analysis with Bioconductor (OSCA).